Ring deconvolution microscopy: exploiting symmetry for efficient spatially varying aberration correction.

Abstract

The most ubiquitous form of aberration correction for microscopy is deconvolution; however, deconvolution relies on the assumption that the system's point spread function is the same across the entire field of view. This assumption is often inadequate, but space-variant deblurring techniques generally require impractical amounts of calibration and computation. We present an imaging pipeline that leverages symmetry to provide simple and fast spatially varying deblurring. Our ring deconvolution microscopy method utilizes the rotational symmetry of most microscopes and cameras, and naturally extends to sheet deconvolution in the case of lateral symmetry. We derive theory and algorithms for ring deconvolution microscopy and propose a neural network based on Seidel aberration coefficients as a fast alternative. We demonstrate improvements in speed and image quality as compared to standard deconvolution and existing spatially varying deblurring across a diverse range of microscope modalities, including miniature microscopy, multicolor fluorescence microscopy, multimode fiber micro-endoscopy and light-sheet fluorescence microscopy. Our approach enables near-isotropic, subcellular resolution in each of these applications.