Resolving the kinetics of an ensemble of muscle myosin motors via a temperature-dependent fitting procedure.

Abstract

A data fitting procedure is devised and thoroughly tested to provide self-consistent estimates of the relevant mechanokinetic parameters involved in a plausible scheme underpinning the output of an ensemble of myosin II molecular motors mimicking the contraction of skeletal muscle. The method builds on a stochastic model accounting for the force exerted by the motor ensemble operated both in the low and high force-generating regimes corresponding to different temperature ranges. The proposed interpretative framework is successfully challenged against simulated data, meant to mimic the experimental output of a one-dimensional synthetic nanomachine powered by pure muscle myosin isoforms.