Eclipse: a Python package for alignment of two or more nontargeted LC-MS metabolomics datasets.

Hitchcock, Daniel S; Krejci, Jesse N; Sturgeon, Chloe E; Dennis, Courtney A; Jeanfavre, Sarah T; Avila-Pacheco, Julian R; Clish, Clary B · Bioinformatics · 2025

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Abstract

Nontargeted LC-MS (liquid chromatography-tandem mass spectrometry) metabolomics datasets contain a wealth of information but present many challenges during analysis and processing. Often, two or more independently processed datasets must be aligned to form a complete dataset, but existing software does not fully meet our needs. For this, we have created an open-source Python package called Eclipse. Eclipse uses a novel graph-based approach to handle complex matching scenarios that arise from n > 2 datasets. Eclipse is open source (https://github.com/broadinstitute/bmxp) and can be installed via "pip install bmxp."

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