FLIM-FRAPP: near-simultaneous characterization of multi-scale polymer dynamics <i>via</i> fluorescence microscopy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 40388182.
- Also identified by DOI 10.1039/d5sm00260e.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Characterizing multi-scale dynamics of polymers is crucial to inform property-process relationships, but there is an outstanding gap between accessibility and comprehensive data collection. To this end, we present a new multi-scale characterization method, FLIM-FRAPP, a near-simultaneous, hybrid approach between fluorescence lifetime measurement (FLIM) and fluorescence recovery after patterned photobleaching (FRAPP) to probe the glass transition temperature and self-diffusion coefficient of a model entangled copolymer, poly(methyl acrylate-<i>stat</i>-methyl methacrylate) (P(MA-<i>stat</i>-MMA)). Our investigation reveals opportunities and challenges concerning the nature of fluorescence characterization techniques for the exploration of polymer dynamics.