Active transport enables protein condensation in cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 40408482.
- Also identified by DOI 10.1126/sciadv.adv7875 and PMC identifier 12101484.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Multiple factors drive biomolecular condensate formation. In plants, condensation of the transcription factors AUXIN RESPONSE FACTOR 7 (ARF7) and ARF19 attenuates response to the plant hormone auxin. Here, we report that actin-mediated movement of cytoplasmic ARF condensates enhances condensation. Coarse-grained molecular simulations of active polymers reveal that applied forces drive the associations of macromolecules to enhance phase separation while giving rise to dense phases that preferentially accumulate motile molecules. Our study highlights how molecular motility can drive phase separation, with implications for motile condensates while offering insights into cellular mechanisms that can regulate condensate dynamics.
Medical subject headings
- Arabidopsis Proteins
- Transcription Factors
- Biomolecular Condensates