M-band wavelet-based multi-view clustering of cells.

Abstract

Wavelet analysis has been recognized as a widely used and promising tool in the fields of signal processing and data analysis. However, the application of wavelet-based method in single-cell RNA sequencing (scRNA-seq) data is little known. Here, we present M-band wavelet-based scRNA-seq multi-view clustering of cells (WMC). We applied for integration of M-band wavelet analysis and uniform manifold approximation and projection (UMAP) to a panel of single cell sequencing datasets by breaking up the data matrix into an approximation or low resolution component and M-1 detail or high resolution components. Our method is armed with multi-view clustering of cell types, identity, and functional states, enabling missing cell types visualization and new cell types discovery. Distinct to standard scRNA-seq workflow, our wavelet-based approach is a new addition to uncover rare cell types with a fine resolution.