Multi-objective computational optimization of human 5' UTR sequences.

Yamada, Keisuke; Suga, Kanta; Abe, Naoko; Hashimoto, Koji; Tsutsumi, Susumu; Inagaki, Masahito; Hashiya, Fumitaka; Abe, Hiroshi et al. · Brief Bioinform · 2025

basic_science · Level V

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Abstract

The computational design of messenger RNA (mRNA) sequences is a critical technology for both scientific research and industrial applications. Recent advances in prediction and optimization models have enabled the automatic scoring and optimization of $5^\prime $ UTR sequences, key upstream elements of mRNA. However, fully automated design of $5^\prime $ UTR sequences with more than two objective scores has not yet been explored. In this study, we present a computational pipeline that optimizes human $5^\prime $ UTR sequences in a multi-objective framework, addressing up to four distinct and conflicting objectives. Our work represents an important advancement in the multi-objective computational design of mRNA sequences, paving the way for more sophisticated mRNA engineering.

Medical subject headings