Force transmission through the inner kinetochore is enhanced by centromeric DNA sequences.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 40439003.
- Also identified by DOI 10.7554/eLife.105150 and PMC identifier 12121997.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Previously, we reconstituted a minimal functional kinetochore from recombinant <i>Saccharomyces cerevisiae</i> proteins that was capable of transmitting force from dynamic microtubules to nucleosomes containing the centromere-specific histone variant Cse4 (Hamilton et al., 2020). This work revealed two paths of force transmission through the inner kinetochore: through Mif2 and through the Okp1/Ame1 complex (OA). Here, using a chimeric DNA sequence that contains crucial centromere-determining elements of the budding yeast point centromere, we demonstrate that the presence of centromeric DNA sequences in Cse4-containing nucleosomes significantly strengthens OA-mediated linkages. Our findings indicate that centromeric sequences are important for the transmission of microtubule-based forces to the chromosome.
Medical subject headings
- Kinetochores
- Centromere
- Saccharomyces cerevisiae
- DNA, Fungal