Pan-viral ORFs discovery using massively parallel ribosome profiling.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 40504907.
- Also identified by DOI 10.1126/science.ado6670 and PMC identifier 12459998.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Defining viral proteomes is crucial to understanding viral life cycles and immune recognition but the landscape of translated regions remains unknown for most viruses. We have developed massively parallel ribosome profiling (MPRP) to determine open reading frames (ORFs) across tens of thousands of designed oligonucleotides. MPRP identified 4208 unannotated ORFs in 679 human-associated viral genomes. We found viral peptides originating from detected noncanonical ORFs presented on class-I human leukocyte antigen in infected cells and hundreds of upstream ORFs that likely modulate translation initiation of viral proteins. The discovery of viral ORFs across a wide range of viral families-including highly pathogenic viruses-expands the repertoire of vaccine targets and reveals potential cis-regulatory sequences.
Medical subject headings
- Open Reading Frames
- Ribosome Profiling
- Ribosomes
- Viral Proteins
- Viruses