A distinct LHCI arrangement is recruited to photosystem I in Fe-starved green algae.

Liu, Helen W; Khera, Radhika; Grob, Patricia; Gallaher, Sean D; Purvine, Samuel O; Nicora, Carrie D; Lipton, Mary S; Niyogi, Krishna K et al. · Proc Natl Acad Sci U S A · 2025

basic_science · Level V

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Abstract

Iron (Fe) availability limits photosynthesis at a global scale where Fe-rich photosystem (PS) I abundance is drastically reduced in Fe-poor environments. We used single-particle cryoelectron microscopy to reveal a unique Fe starvation-dependent arrangement of light-harvesting chlorophyll (LHC) proteins where Fe starvation-induced TIDI1 is found in an additional tetramer of LHC proteins associated with PSI in <i>Dunaliella tertiolecta</i> and <i>Dunaliella salina</i>. These cosmopolitan green algae are resilient to poor Fe nutrition. TIDI1 is a distinct LHC protein that co-occurs in diverse algae with flavodoxin (an Fe-independent replacement for the Fe-containing ferredoxin). The antenna expansion in eukaryotic algae we describe here is reminiscent of the iron-starvation induced (<i>isiA-</i>encoding) antenna ring in cyanobacteria, which typically co-occurs with <i>isiB</i>, encoding flavodoxin. Our work showcases the convergent strategies that evolved after the Great Oxidation Event to maintain PSI capacity.

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