Multiplexed imaging of radionuclides.

Abstract

Nuclear imaging provides non-invasive and near-quantitative insight into the biodistribution of radiolabelled compounds, and it does so with exceptional sensitivity and practically unlimited penetration depth. These properties make nuclear imaging highly valuable for monitoring the pharmacokinetics, biodistribution and in vivo stability of therapeutics. Moreover, the diversity of radioactive probes allows for detailed insight into cell dynamics, metabolism, epigenetics and other biological processes. However, nuclear imaging remains largely limited to single-tracer studies, or to the sequential imaging of each tracer. Tracking only a single probe or compound at a time limits the insight that can be gained. Here we discuss the applications and clinical feasibility of established and upcoming strategies for the simultaneous imaging of multiple radiotracers.