Human amniotic epithelial stem cells-conditioned media (hAECs-CM) reduces inflammation and promotes wound healing in a mouse burn model.

Abstract

This study aimed to assess the impact of human amniotic epithelial stem cells-conditioned medium (hAECs-CM) on the healing of burn wounds and the reduction of inflammation in a mouse model of deep second degree burns. The findings indicated that hAECs-CM markedly enhanced the primary metrics of burn wound healing within a brief duration of 14 days. The rate of wound healing was substantially greater than that of the control group following 21 days of treatment with daily administration of hAECs-CM. The CD31 concentration was markedly elevated in burn tissues treated with hAECs-CM, whereas the myeloperoxidase (MPO) level was reduced. Simultaneously, the antibacterial efficacy of hAECs-CM was assessed, indicating that hAECs-CM may alleviate infections generated by burns. HE and Masson staining results indicated that the treatment markedly diminished inflammation and collagen deposition relative to the model group, while both connective tissue and neovascularization were enhanced in the dermis of the hAECs-CM-BI group. In conclusion, the utilization of hAECs-CM may expedite the wound healing process and mitigate burn infections.