Caffeine Habituation, Not CYP1A2 Genotype, Modulates the Acute Effect of Caffeine on Exercise-Induced Hemostatic Responses in Adults with Obesity.

Abstract

This study aimed to investigate how genotype and caffeine habituation influence the acute effects of caffeine ingestion on exercise-induced hemostatic responses in individuals with obesity. Using a randomized, double-blind, placebo-controlled crossover design, 40 physically inactive young men with obesity (age, 22.2 ± 2.3 yr; body mass index, 34.1 ± 2.7 kg·m -2 ) completed two moderate-to-high-intensity concurrent exercise sessions following ingestion of caffeine (3 mg·kg -1 ) or placebo. Blood samples were collected at baseline, after exercise, and after 60 min of recovery. Statistical analysis was performed by repeated-measures multivariate analysis of variance. Acute exercise increased platelet count and aggregation, fibrinogen, F1 + 2, tPA antigen, D-dimer, and clot lysis time, regardless of genotype or caffeine habituation status ( P < 0.05). PAI-1 antigen remained unchanged after exercise ( P > 0.05) but decreased following recovery ( P < 0.01). Caffeine resulted in a greater increase in platelet aggregation, fibrinogen, F1 + 2, and clot lysis time, alongside a blunted increase in tPA antigen levels post-exercise in naïve consumers ( P < 0.05). In contrast, habitual caffeine consumers exhibited a mitigated increase in clot lysis time and a greater post-recovery reduction in PAI-1 antigen following caffeine ingestion ( P < 0.001). Caffeine's impact on hemostatic responses to exercise was unaffected by genotype ( P > 0.05). Moderate-to-high-intensity concurrent exercise induces a transient prothrombotic state in physically inactive individuals with obesity. Acute caffeine supplementation at a moderate dose modulates the hemostatic responses depending on caffeine habituation status rather than CYP1A2 genotype: it exacerbates the prothrombotic response in naïve consumers but attenuates it in habitual consumers.