A Nanobiocatalyst-Driven Hybrid System for Efficient and Sustainable Hydrogen Production via Electron Flow Optimization.

Yang, Linlin; Dong, Yizhe; Zhao, Dong; Li, Xiangyu; He, Jiajie; Lai, Tin Pou; Zhou, Enze; Ueki, Toshiyuki et al. · Adv Mater · 2025

basic_science · Level V

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Abstract

Biohydrogen production offers a promising pathway for developing clean and renewable energy sources. However, its practical application has been hindered by low efficiency and sustainability issues. Here, it is introduced an energy-efficient and output-sustainable hybrid system (LPBC/CH system) for biohydrogen production by integrating self-assembling intermetallic (L1<sub>0</sub>) FePt@polypyrrole nanobiocatalysts (LPBC) with Clostridium pasteurianum. The engineered LPBC, characterized by optimal atomic structures and defined electronic properties, demonstrates robust transcriptional enhancement efficiency and biocatalytic capability, leading to an ≈103% increase in hydrogen production rate and a 57% enhancement in hydrogen yield to the bare C. pasteurianum system. Within the LPBC/CH system, the nanobiocatalysts target NADH and [FeFe] hydrogenase, triggering efficient tandem biocatalytic reactions for proton reduction. Notably, the LPBC achieves sustained performance for at least 30 days - a benchmark unmatched by other reported nanobiocatalysts. This study not only advances the frontiers of biohydrogen production but also establishes a universal framework for constructing hybrid systems with superior efficiency and sustainability.