PEANUT: Pathway Enrichment Analysis through Network UTilization.

Pickholz Berliner, Yair; Sharan, Roded · Bioinformatics · 2025

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Abstract

Pathway enrichment analysis is a fundamental technique in bioinformatics for interpreting gene expression data to pinpoint biological pathways associated with specific conditions or diseases. We introduce Pathway Enrichment Analysis through Network UTilization (PEANUT), a web-based tool for pathway enrichment analysis that enhances traditional pipelines by integrating network propagation computations within a network of protein-protein interactions (PPIs). By diffusing gene expression scores through the PPI network, PEANUT amplifies the signals of connected sets of genes, thereby improving the detection of relevant pathways. The tool is accessible as an open-source web application at https://peanut.cs.tau.ac.il/. The source code is available at https://github.com/Yapibe/PEANUT with a permanent identifier (DOI: https://doi.org/10.5281/zenodo.15184862).

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