Rotation-invariance is essential for accurate detection of spatially variable genes in spatial transcriptomics.

Su, Haohao; Cui, Yuehua · Nat Commun · 2025

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Abstract

In spatial transcriptomics, tissue samples are randomly positioned. Rotation-sensitive methods can lead to unreliable spatially variable gene (SVG) detection. We highlight their inherent technical pitfalls and discuss strategies for rotation-invariant methods, enhancing the robustness of SVG detection.