Strontium and Tannic Acid-Modified Eggshell-Derived Nanoparticles Promote Wound Healing in Poly(Lactic Acid)/Gelatin-Based Nanofibrous Dressings.

Abstract

Wound healing may be impaired by excessive bleeding, bacterial colonization, slower angiogenesis, and poor cell proliferation, which necessitate the advent of innovative hemostatic, antibacterial, and pro-angiogenic dressings. The objective of this research is to synthesize strontium-substituted tannic acid-modified eggshell-based nanoparticles (ECa-TA-Sr NPs) and exploit them to endow multifunctionality to poly(lactic acid)/gelatin (PG) fibers. Physical and chemical properties of unmodified and modified NPs, as well as dressings, are evaluated with different types of assays in vitro. Hemostatic ability as well as wound healing ability of dressings is discerned in a rat liver trauma model and a full-thickness excisional defect model in vivo. Dressings loaded with ECa-TA-Sr NPs exhibit antibacterial activity against E. coli and S. aureus, anti-inflammatory ability, angiogenic ability, rapid hemostatic ability, and faster wound closure than that of the PG fibers. Therefore, ECa-TA-Sr NPs derived from the eggshell can enable multifunctionality to dressings and may also be worthy for future investigations.