Vaccination with Acinetobacter baumannii adhesin Abp2D provides protection against catheter-associated urinary tract infection.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 40783485.
- Also identified by DOI 10.1038/s41467-025-62402-9 and PMC identifier 12335481.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Catheter-associated urinary tract infections (CAUTIs) contribute greatly to the burden of healthcare-associated infections. Acinetobacter baumannii is a Gram-negative bacterium with high levels of antibiotic resistance that is of increasing concern as a CAUTI pathogen. A. baumannii expresses fibrinogen-binding adhesins (Abp1D and Abp2D) that mediate biofilm formation on catheters, which become coated with fibrinogen upon insertion. Here we develop a protein subunit vaccine against the Abp1D and Abp2D receptor binding domains (RBD) and show that vaccination significantly reduces bacterial titers in a female mouse model of CAUTI. We further demonstrate that immunity to Abp2D<sub>RBD</sub> alone is sufficient for protection. Mechanistically, we define the B cell response to Abp2D<sub>RBD</sub> vaccination, demonstrate that passive immunization with Abp2D<sub>RBD</sub>-immune serum transfers immunity to naïve mice, and show that Abp2D<sub>RBD</sub>-immune serum inhibits bacterial binding to fibrinogen-coated catheters. This work represents an antibiotic-sparing strategy for the prevention of A. baumannii CAUTI which has an important role in the global fight against antimicrobial resistance.
Medical subject headings
- Acinetobacter baumannii
- Urinary Tract Infections
- Adhesins, Bacterial
- Acinetobacter Infections
- Catheter-Related Infections
- Bacterial Vaccines