Expression of small leucine-rich proteoglycans in the medial meniscus posterior horn associated with medial meniscus posterior root tear.

Abstract

Small leucine-rich proteoglycans (SLRPs) play a regulatory role in collagen fibril growth and organization. Medial meniscus posterior root tear (MMPRT) causes the loss of medial meniscus (MM) function and the rapid degradation of the articular joint. In this study, we aimed to evaluate the expression levels of SLRPs in the MM posterior horn in relation to MMPRT. MM tissue samples were obtained from patients with MMPRT (mean age: 72.6, n = 5) and control patients with bone and soft tissue tumors (mean age: 69.8, n = 5), all of whom underwent knee arthroplasty. Patients with MMPRT who underwent surgery within three months of the injury were included. Quantitative real-time PCR was performed to evaluate the expression levels of transforming growth factor-β1 (TGFB1) and SLRPs in the cultured MM posterior horn cells. Additionally, expression of TGF-β1 and SLRPs proteins was analyzed using immunohistochemical staining and quantified using image analysis. Quantitative real-time PCR analysis revealed that the expression levels of SLRPs were higher in the MMPRT group than in the control group. Significantly elevated expression levels of TGFB1 (3.2-fold), lumican (4.2-fold), and fibromodulin (3.8-fold) were observed in the MMPRT group compared to those in the control group. Similarly, immunohistochemistry revealed more TGF-β1-, lumican-, and fibromodulin-positive cells in the MMPRT group compared to those in the control group. This study is the first to demonstrate elevated expression of SLRPs, especially lumican and fibromodulin, in menisci of patients with MMPRT, suggesting that these proteins could serve as potential biomarkers or therapeutic targets for MMPRT treatment.