Quantitative Analysis of Glutathione Utilizing Highly Luminescent Gold Nanoclusters.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 40977580.
- Also identified by DOI 10.1002/jbm.a.37988.
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Abstract
Glutathione (GSH), a pivotal regulator of cellular redox homeostasis, requires precise monitoring for clinical diagnostics. This work develops bovine serum albumin-templated gold nanoclusters (BSA-AuNCs) through in situ chloroauric acid reduction, exhibiting orange fluorescence (λ<sub>em</sub> = 610 nm) with 35.8% quantum yield. Systematic evaluation of pH-dependent and time-resolved quenching behavior revealed optimal GSH detection at physiological pH 7.4 within 3 min. The nanozyme demonstrated linear responsivity from 10 nM to 3 μM (Limit of Detection, LOD = 8.7 nM) and validated applicability in biological matrices (urine/serum) with 98.0%-103.3% recovery rates. This photoluminescent platform enables reliable GSH biomarker quantification, advancing point-of-care testing for oxidative stress disorders.
Medical subject headings
- Glutathione
- Gold
- Metal Nanoparticles