Scaling DNA synthesis with a microchip-based massively parallel synthesis system.
basic_science · Level V
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- Record sourced from PubMed, PMID 41034496.
- Also identified by DOI 10.1038/s41587-025-02844-0.
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Abstract
Current high-throughput DNA synthesis technologies use intricate chip and microfluidic systems to produce large-scale synthetic oligonucleotides but with low concentration and limited compatibility for long DNA assembly. Here we report a massive-in-parallel synthesis system, with an 'identification-sorting-synthesis-recycling' iteration mechanism applied to microchips for high-throughput DNA synthesis. This approach increases DNA product concentration by four to six orders of magnitude and simplifies downstream processes for large-scale gene synthesis.