Left-handed DNA for efficient highly multiplexed imaging at single-protein resolution.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 41038860.
- Also identified by DOI 10.1038/s41467-025-64228-x and PMC identifier 12491495.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Multiplexed super-resolution microscopy enables spatial proteomics at single-protein resolution, but current methods often depend on secondary labels, complicating implementation and limiting throughput. We introduce a streamlined approach that combines speed-optimized DNA-PAINT sequences with their mirror-image analogs (left-handed DNA), enabling rapid and efficient 12-plex imaging. Validated on synthetic and cellular benchmarks, our method maps dense neuronal interactomes in 3D with 15 nm spatial resolution across a 200 × 200 µm<sup>2</sup> field of view.
Medical subject headings
- DNA
- Proteomics
- Proteins