Activatable fluorescent ratiometric probes for early diagnosis and prognostic assessment of acute kidney injury.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 41124265.
- Also identified by DOI 10.1126/sciadv.aea1654 and PMC identifier 12542964.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Early and accurate diagnosis of acute kidney injury (AKI) is crucial for clinical treatment. However, most existing fluorescent probes are prone to background interference and limited renal targeting. We developed a ratiometric nanoprobe (RP-SC) that synergistically responded to H<sub>2</sub>O<sub>2</sub> and targeted kidney injury molecule-1 (KIM-1). In AKI kidneys, RP-SC was hydrolyzed and slowly released encapsulated Hcy-BOH and Cy-Dopa. The H<sub>2</sub>O<sub>2</sub> level was semi-quantitatively analyzed by fluorescence ratio (<i>F</i><sub>Hcy-BOH</sub>/<i>F</i><sub>Cy-Dopa</sub>). RP-SC exhibited prominent renal fluorescence in AKI mice, making it possible to accurately diagnose AKI and dynamically monitor renal function for up to 60 hours. RP-SC could monitor the process of AKI in vivo and provide an earlier warning of AKI than traditional strategies, subsequently evaluating the recovery of renal function after <i>N</i>-acetyl cysteine (NAC) treatment. Our study confirmed the ability of RP-SC for longitudinal monitoring of renal function in vivo by dual-targeting capacity, providing an effective tool for accurate diagnosis of early AKI while tracking the dynamic process of AKI treatment.
Medical subject headings
- Acute Kidney Injury
- Fluorescent Dyes