Ultra-mild bisulfite outperforms existing methods for 5-methylcytosine detection with low input DNA.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 41233368.
- Also identified by DOI 10.1038/s41467-025-66033-y and PMC identifier 12615686.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
We present Ultra-Mild Bisulfite Sequencing (UMBS-seq), a method for 5-methylcytosine (5mC) detection that minimizes DNA degradation and background noise. UMBS-seq outperforms conventional bisulfite and enzymatic methyl-sequencing (EM-seq) methods in library yield, complexity, and conversion efficiency when applied to low-input DNA samples. In particular, its effectiveness with low-input cell-free DNA (cfDNA) and hybridization-based target capture highlights its potential for clinical applications, including 5mC biomarker detection and early disease diagnosis.
Medical subject headings
- 5-Methylcytosine
- Sulfites
- Sequence Analysis, DNA
- High-Throughput Nucleotide Sequencing
- DNA