Cyclin CLB2 mRNA localization and protein synthesis link cell cycle progression to bud growth.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 41298462.
- Also identified by DOI 10.1038/s41467-025-66623-w and PMC identifier 12749197.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Clb2 is a conserved B-type cyclin essential for mitotic progression in Saccharomyces cerevisiae, with expression tightly regulated at transcriptional and proteolytic levels. However, it remains unclear whether Clb2 protein synthesis is regulated and responsive to cell growth. Here, we show that CLB2 mRNA localizes to the bud via the She2/She3 complex, while Clb2 protein accumulates in the mother nucleus. This mRNA localization enhances translation without affecting protein localization. A structured RNA element, a ZIP-code, is located within the coding sequence and is required, but not sufficient, for both mRNA transport and protein expression. Mutation of this ZIP code disrupts mRNA localization, reduces Clb2 synthesis, increases budded phase duration and daughter cell size. In wild-type cells, Clb2 protein levels scale with bud growth, a coupling lost in ZIP code mutants. These findings reveal a mechanism by which mRNA localization and translation are coordinated to link cell growth with cell cycle progression.
Medical subject headings
- Saccharomyces cerevisiae Proteins
- Saccharomyces cerevisiae
- RNA, Messenger
- Cyclin B
- Protein Biosynthesis
- Cell Cycle