Visualizing dynamics of membrane rafts on live cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 41313761.
- Also identified by DOI 10.1126/sciadv.adv7001 and PMC identifier 12662213.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Membrane rafts are cellular portals to external stimuli that trigger signaling cascades for sophisticated yet remarkable biochemical activities. Visualization of the topographic evolution of membrane rafts remains unreported on live cells due to the nanosized and dynamic nature. Here, an imaging strategy involving atomic force microscopy and Hadamard product is developed to unveil membrane-raft features. Michigan Cancer Foundation-7 (MCF-7) cells were subjected to fibrinogen or manganese(II) (Mn<sup>2+</sup>)/resveratrol, both of which are ligands of integrin α<sub>V</sub>β<sub>3</sub> embedded within membrane rafts; the former promotes metastasis, and the latter enables apoptosis. MCF-7 cellular membranes responded to the two stimulants markedly different. The size, height, spatiotemporal trajectory, and persistent time of ligand-activated nanodomains are revealed. This approach opens up a visualized platform toward the understanding of activation-associated signaling cascades.
Medical subject headings
- Membrane Microdomains