The evolving landscape of precise DNA insertion in plants.
review · Level V
Where this comes from
- Record sourced from PubMed, PMID 41330932.
- Also identified by DOI 10.1038/s41467-025-66715-7 and PMC identifier 12672739.
- Licence recorded as CC BY-NC-ND.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Precise DNA insertion into plant genomes is central to advancing crop improvement and synthetic biology. CRISPR-Cas systems have enabled programmable DNA integration using tools such as gene targeting (GT), prime editing (PE), and recombinase- or transposase-based platforms. These tools are transitioned from theoretical concepts to practical applications, supporting applications like in-locus protein tagging, regulatory element engineering, and multi-gene stacking. Key challenges persist, such as inefficient large-fragment insertion, delivery barriers, and regulatory hurdles. This review traces the evolution from random to CRISPR-Cas-based systems, analyzes current limitations, and discusses emerging solutions paving the way for predictable DNA insertion in modern plant biotechnology.
Medical subject headings
- CRISPR-Cas Systems
- DNA, Plant
- Plants
- Mutagenesis, Insertional