Molecular-scale isotropic 3D super-resolution microscopy via interference localization.
basic_science · Level V
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- Record sourced from PubMed, PMID 41331140.
- Also identified by DOI 10.1038/s41592-025-02911-z.
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Abstract
Three-dimensional (3D) nanoscale imaging reveals the detailed morphology of subcellular structures; however, conventional single-molecule localization microscopy is constrained by limited axial resolution. Here we introduce ROSE-3D, an interferometric localization approach that enables isotropic 3D super-resolution imaging with uniform performance across the entire depth of field. Compared with conventional astigmatism-based methods, ROSE-3D improves lateral localization precision by 2-6 times and axial precision by 3.5-8 times over a depth of field of approximately 1.2 μm. Leveraging its multicolor and whole-cell imaging capabilities, ROSE-3D resolves, in situ, the nanoscale organization of nuclear lamins and the assemblies of mitochondrial fission-related protein DRP1. These results establish ROSE-3D as a powerful tool for interrogating nanoscale cellular architecture.
Medical subject headings
- Imaging, Three-Dimensional
- Single Molecule Imaging