Evaluating MINFLUX experimental performance in silico.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 41353189.
- Also identified by DOI 10.1038/s41467-025-66952-w and PMC identifier 12783130.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
MINFLUX is a super-resolution microscopy technique with remarkable resolution for imaging and tracking. Its optical resolution is well understood under idealized conditions, but because of system complexity and experimental imperfections, actual performance can be much worse. Here we present SimuFLUX, a comprehensive and realistic simulator for MINFLUX. We use it to investigate performance limits caused by fluorophore dynamics, background, estimators, and misalignment, and to simulate experiments to optimize parameters and assess feasibility.