A study on the diagnostic significance and biological roles of lncRNA DUXAP8 in osteoporosis.

Abstract

BackgroundOsteoporosis (OP) is a progressive metabolic disorder resulting from an uncoupling of bone formation and breakdown processes.ObjectivesThis study mainly explored the effects of lncRNA DUXAP8 on the biological functions and osteogenic potential of hBMSCs (human bone marrow mesenchymal stem cells).MethodsThis study enrolled 35 OP patients and 35 healthy individuals. The expression profiles of lncRNA DUXAP8 and miR-24-3p in serum and cells were analyzed using RT-qPCR. Additionally, osteogenic marker expression at mRNA and protein levels was assessed. The proliferation ability of hBMSCs was evaluated by CCK-8 assay, with cell motility and invasiveness assessed by Transwell assay. The regulatory relationship between lncRNA DUXAP8 and miR-24-3p was verified by Dual-Luciferase.ResultsSerum lncRNA DUXAP8 levels were reduced in OP patients, with an AUC of 0.967 (95% CI: 0.931-1.000), sensitivity 0.914, and specificity 0.943. Inhibition of lncRNA DUXAP8 decreased osteogenic markers (mRNA/protein) and hBMSCs proliferation, migration, invasion, while overexpression had opposite effects. LncRNA DUXAP8 targeted miR-24-3p, and miR-24-3p overexpression reversed the promoting effects of oe-DUXAP8 on hBMSCs functions.ConclusionLncRNA DUXAP8 in the serum of OP patients is decreased. LncRNA DUXAP8 has a high diagnostic value for patients with OP. Overexpression of lncRNA DUXAP8 may enhance hBMSCs' proliferative and osteogenic potential by targeting miR-24-3p.