Equilibrium scaling of phase separated elastin-like polypeptides for engineered condensates.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 41367279.
- Also identified by DOI 10.1039/d5sm00873e.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
The interaction parameter of the Flory-Huggins free-energy of two phase separating components was experimentally determined and fit to a semi-empirical model. This model captures the temperature and chain length dependence, quantitatively describing phase equilibria and critical temperatures in elastin-like polypeptide systems of <i>VPGVG</i> pentameric repeats. We show temperature driven turbidity measurements of phase separation will recapitulate equilibrium through a simple temperature translation. This integrates both experiment and theory of liquid-liquid phase separation, offering a predictive tool for the ability to engineer ELP condensates and coacervates by tuning number of pentamers, temperature and concentration.