Fractal-shaped droplet microfluidics for highly scalable cell mechanoporation.

Sung, Myungsuk; Jing, Dalei; Joo, Byeongju; Im, Sungbin; Kim, You-Jeong; Sui, Yi; Chung, Aram J · Lab Chip · 2026

basic_science · Level V

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Abstract

Emerging non-viral gene delivery platforms provide alternatives to viral methods. However, they remain limited in scalability and efficiency for clinical translation. We present a fractal-shaped droplet microfluidic system that achieves approximately 98% efficiency and 80% viability at throughputs exceeding 10<sup>7</sup> cells per min, enabling efficient, large-scale, and clinically relevant cell engineering.

Medical subject headings