Preclinical Evaluation of <sup>64</sup>Cu-LNTH-1363S to Detect Cardiac FAP-Positive Fibroblasts Using PET Imaging.
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- Record sourced from PubMed, PMID 41381234.
- Also identified by DOI 10.2967/jnumed.125.270555 and PMC identifier 12955528.
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Abstract
Fibroblast activation protein-expressing (FAP+) fibroblasts are central mediators of cardiac remodeling after injury or disease. The aim of this study was to evaluate a new PET tracer, <sup>64</sup>Cu-LNTH-1363S, to detect FAP+ fibroblasts in myocardial infarction (MI) mouse models. <b>Methods:</b> The permanent left anterior descending coronary artery ligation and the echocardiography-guided ischemia reperfusion models were used to assess the targeting efficiency of <sup>64</sup>Cu-LNTH-1363S. Competitive receptor blocking and autoradiography studies were performed to confirm the in vivo targeting specificity. <b>Results:</b> <sup>64</sup>Cu-LNTH-1363S demonstrated sensitive and specific detection of FAP+ cells in the 2 MI models. Kinetic modeling data showed that the distribution volume was higher in the infarct zone than in the remote myocardium. <b>Conclusion:</b> This study establishes the feasibility of <sup>64</sup>Cu-LNTH-1363S for noninvasive detection of FAP+ fibroblasts in MI mouse models and provides preclinical data to support further investigation of this process in humans after various forms of cardiac injury.
Medical subject headings
- Positron-Emission Tomography
- Fibroblasts
- Copper Radioisotopes
- Serine Endopeptidases
- Gelatinases
- Membrane Proteins
- Myocardium