Global implications of a low soil moisture threshold for microbial hydrogen uptake.

Abstract

The impact of increasing anthropogenic hydrogen (H₂) emissions on Earth's radiative balance depends on the soil microbial H₂ sink-the largest and most uncertain term in the global H₂ budget. Soil moisture is a primary but poorly quantified control regulating the soil sink. Here, we assess the sensitivity of microbial H₂ oxidation to soil moisture in laboratory experiments with temperate and arid soils spanning distinct textures. We report H₂ oxidizer activity down to -70 to -100 MPa water potentials across soils, which are among the driest conditions reported for microbial activity and are much drier than assumed in global simulations of H₂. Using genome-resolved meta-omics, we link H₂ oxidation dynamics in temperate soils to specific desiccation-adapted microbial taxa that contribute differentially to H₂ uptake along the moisture gradient. Through global simulations, we show that our observationally constrained drier moisture threshold increases the contribution of arid and semi-arid regions for soil H₂ uptake by 4-7 percentage points (pp), while decreasing the contribution of temperate and continental regions (-7 pp). Our results highlight the importance of H₂ uptake under extreme hydrological conditions, particularly the roles of desertification, dryland expansion, and H₂-oxidizer ecophysiology in modulating long-term changes in H₂ uptake.