SPOROCYTELESS/NOZZLE cooperates with MADS-domain transcription factors to regulate an auxin-dependent network controlling Megaspore-Mother-Cell differentiation.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 41392078.
- Also identified by DOI 10.1038/s41467-025-67343-x and PMC identifier 12820212.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
The formation of the female gamete is a complex developmental process that begins with the differentiation of the Megaspore Mother Cell (MMC) within the ovule. SPOROCYTELESS/NOZZLE (SPL/NZZ) is the principal regulator of the MMC formation, as mutations in the SPL/NZZ gene lead to the failure of the MMC differentiation. Nonetheless, the SPL/NZZ-dependent regulatory pathway governing the MMC development remains largely unknown. Using a multi-omics approach, we identify direct SPL/NZZ targets and their downstream network. We discover that SPL/NZZ interacts with ovule-identity MADS-domain transcription factor complexes to regulate the expression of common target genes. By integrating the omics data with the analysis of either complementation or mutant lines, we describe a comprehensive regulatory mechanism in which SPL/NZZ controls the differentiation of the MMC by acting on an auxin-dependent downstream network.
Medical subject headings
- Arabidopsis Proteins
- Indoleacetic Acids
- Cell Differentiation
- Arabidopsis
- MADS Domain Proteins
- Ovule
- Transcription Factors