An <i>in vitro</i> organ-on-chip model for studying neuron-keratinocyte interactions in sensory response through electrophysiology.

Bessy, Thomas; Martinez, Anthony; Baquerre, Camille; Grégoire, Corinne; Batut, Aurélie; Berthelin, Aurore; Dubuisson, Louise; Teluob, Séverine et al. · Lab Chip · 2026

basic_science · Level V

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Abstract

This study introduces a human-relevant <i>in vitro</i> model using iPSC-derived sensory neurons and keratinocytes in MEA-integrated microfluidic chips. Neurons expressed nociceptor markers, showed TRPV activity, and formed contacts with keratinocytes. Stimuli evoked electrophysiological responses, highlighting neuron-keratinocyte interactions relevant to pruritus, pain, and skin disorders, supporting therapeutic development.

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