Closed-loop optogenetic control of cell biology enables outcome-driven microscopy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 41430058.
- Also identified by DOI 10.1038/s41467-025-67848-5 and PMC identifier 12852791.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Smart microscopy is transforming biological imaging by integrating real-time analysis with adaptive acquisition to enhance imaging efficiency. Whereas many emerging implementations are event-driven and focus on on-demand data acquisition to reduce phototoxicity, we here present 'outcome-driven' microscopy, a framework combining smart microscopy with optogenetics to control cell biological processes and achieve predefined outcomes. We validate this approach using light-based control of cell migration and nucleocytoplasmic transport, demonstrating robust spatiotemporal control of cellular behaviour in single cells and in cell populations.
Medical subject headings
- Optogenetics
- Microscopy