Intraoperative Molecular Imaging Can Detect Large Nerve Perineural Invasion: A Case Report.

Burns, Carleigh R; Mattingly, Aviva S; Ely, Kim; Radevic, Andreja; Meeks, Nicole; Zaidi, Sydea Maria Ahmad; Brown, Brandee; Vasiukov, Georgii et al. · Head Neck · 2026

case_report · Level V

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Abstract

Perineural invasion (PNI) in head and neck squamous cell carcinoma (SCC) results in worse overall survival. Diagnosis requires resection and microscopic evaluation. A 63-year-old male with persistent cT4aN0 p16-positive SCC of the left base of tongue following chemoradiotherapy underwent salvage total glossectomy. Fluorescence-guided imaging of the wound bed was performed with PDE-GEN3 near-infrared (NIR) imaging following infusion of an optically EGFR-targeted antibody, Panitumumab-IRDye800 (pan800). The proximal resected hypoglossal nerve was imaged intraoperatively and demonstrated a strong green fluorescence signal, raising concern for subclinical PNI. Biopsy of the nerve revealed SCC on frozen section analysis. This was re-resected with subsequent proximal margin negative for carcinoma. Postoperatively, ex vivo imaging of the nerve using PDE-GEN3 NIR imaging again demonstrated the presence of pan800 within the initial positive nerve margin, confirming subclinical PNI. This case shows the feasibility of intraoperative fluorescence as a method to help identify subclinical PNI. ClinicalTrials.gov identifier: NCT05945875.

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