Self-driving bioprinting laboratories.

Abstract

The severe shortage of donor organs and limitations of current disease models highlight the urgent need for transformative strategies in tissue engineering (TE) and regenerative medicine (RM). Bioprinting has emerged as a powerful approach for creating functional tissues and organs, yet current workflows remain labor-intensive, variable, and challenging to scale. The convergence of artificial intelligence (AI), advanced bioprinting technologies, robotics, biosensing, and cutting-edge biological methods is catalyzing the development of self-driving bioprinting laboratories-a fully integrated, autonomous, closed-loop system capable of designing, fabricating, maturing, and assessing living tissue constructs, as well as supporting seamless transplantation, with minimal human intervention. By integrating autonomous cellular farming, on-demand bioink formulation, intelligent optical and digital reconstruction platforms, AI-driven bioprinting, intelligent bioreactors, and robotic transplantation within a sterile, interconnected ecosystem, such platforms can continuously learn, adapt, and optimize workflows, enabling standardized, scalable tissue manufacturing and facilitating a seamless transition from bench to bedside. This perspective outlines the foundational technologies, opportunities, and challenges for realizing self-driving bioprinting, envisioning a future where intelligent, automated platforms transform TE and RM into a scalable, predictive, and clinically integrated discipline at the forefront of precision medicine.