Rad51 determines pathway usage in post-replication repair.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 41519855.
- Also identified by DOI 10.1038/s41467-025-68109-1 and PMC identifier 12876063.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Stalled replication forks are processed in post-replication repair by homologous recombination, fork regression, and translesion DNA synthesis. However, the regulation of pathway usage is not fully understood. Rad51 protein maintains genomic stability through its roles in recombination and in protecting stalled replication forks. We report isolation of mutations in Saccharomyces cerevisiae Rad51 that shift post-replication repair from recombination to alternate pathways including mutagenic translesion synthesis. Rad51-E135D and Rad51-K305N show near normal in vitro recombination despite changes in their DNA binding profiles, in particular to dsDNA. The mutants lead to a defect in Rad51 recruitment to stalled forks in vivo as well as a defect in the protection of dsDNA from degradation by Dna2-Sgs1 and Exo1 in vitro. Together, the evidence suggests that Rad51 binding to duplex DNA is critical to control pathway usage at stalled replication forks.
Medical subject headings
- Rad51 Recombinase
- Saccharomyces cerevisiae Proteins
- Saccharomyces cerevisiae
- DNA Replication
- DNA Repair