Bioprinting collagenase-responsive hydrogel for controlled release of cowpea mosaic virus immunotherapy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 41529396.
- Also identified by DOI 10.1088/1758-5090/ae37de and PMC identifier 12848481.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
In this work, we developed a collagenase-responsive hydrogel system to covalently load cancer immunotherapy candidate cowpea mosaic virus (CPMV) using 3D digital light processing bioprinting technology. CPMV was functionalized with norbornene groups (CPMV-NB), which was then bioprinted into hydrogels with 8-arm polyethylene glycol norbornene and a collagenase-cleavable peptide via photoinduced thiol-ene click chemistry. This strategy enabled stable retention of CPMV-NB within the hydrogels and achieved controlled release of CPMV-NB triggered by collagenase. Furthermore, released CPMV-NB retained its immunogenicity to stimulate immune cells.
Medical subject headings
- Comovirus
- Hydrogels
- Collagenases
- Printing, Three-Dimensional
- Immunotherapy