ATAD2 mediates chromatin-bound histone chaperone turnover.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 41557467.
- Also identified by DOI 10.7554/eLife.107582 and PMC identifier 12818868.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
ATAD2, a conserved protein which is predominantly expressed in embryonic stem (ES) cells and spermatogenic cells, emerges as a crucial regulator of chromatin plasticity. Our previous parallel studies conducted in both ES cells and <i>S. pombe</i> highlighted the fundamental role of ATAD2 in facilitating chromatin-bound histone chaperone turnover. Focusing on mouse spermatogenesis, we demonstrate here that ATAD2 regulates the HIRA-dependent localization of H3.3 on the genome and influences H3.3-mediated gene transcription. Moreover, by modulating histone eviction and the assembly of protamines, ATAD2 ensures proper chromatin condensation and genome packaging in mature sperm. Disruption of <i>Atad2</i> function in mice leads to abnormal genome organization in mature spermatozoa. Together, these findings establish a previously overlooked level of chromatin dynamic regulation, governed by ATAD2-controlled histone chaperones binding to chromatin, which defines the balance between histone deposition and removal.
Medical subject headings
- Histone Chaperones
- Chromatin
- ATPases Associated with Diverse Cellular Activities
- Histones
- DNA-Binding Proteins