Nonmonotonic Enhancement of Signal Gain by Outer-Surface Charge in Nanopores: toward Precise Single-Entity Detection.
basic_science · Level V
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- Record sourced from PubMed, PMID 41564870.
- Also identified by DOI 10.1021/acs.nanolett.5c05903.
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Abstract
Nanopore technology is a powerful tool for studying the native structures and properties of single entities. However, it sometimes suffers from insufficient accuracy and a potential false positive rate due to low ionic current signal gain. Herein, we report a methodology whereby the manipulation of nanopore outer-surface (OS) charges enhances the ionic current signal gain, which, in turn, improves detection accuracy in single-entity analysis. The results demonstrate that OS charges enhance the ionic current change value (Δ<i>I</i>) and ionic current change rate (β), two key signals for nanopore single-entity analysis. Both signals exhibit OS charge-dependent optimization thresholds for high accuracy, with Δ<i>I</i> peaking at an OS charge of 1.100 C/m<sup>2</sup> and β peaking at 0.600 C/m<sup>2</sup> (computational modeling framework established in this work). This finding provides valuable guidance for developing and designing nanopore sensors for high-accuracy, single-entity detection.