Quantitative Stimulated Emission Depletion (STED) Microscopy with DNA-Fluorophore Labels.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 41632257.
- Also identified by DOI 10.1021/acsnano.5c21411 and PMC identifier 12918720.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Stimulated emission depletion (STED) microscopy enables super-resolution imaging of complex biological samples in 3D, in large volumes, and live. However, molecular quantification with STED has remained underexplored. Here, we present a straightforward approach for quantitative STED that enables molecule counting. For this purpose, we designed DNA-fluorophore labels that enable signal amplification and allow for reliable intensity-based quantitative imaging. We demonstrate accurate molecule counting on DNA origami. Furthermore, we visualized and quantified EGF receptor monomers and dimers in cells. In summary, we introduce a robust, fast, and easy-to-implement tool for quantitative STED microscopy with single-protein resolution.
Medical subject headings
- DNA
- Fluorescent Dyes