Diagnostic-Mediated Interactive Superlattices Engineering for Threshold-Saltatory Visual Assessment of Tuberculous Meningitis.

Abstract

Nonlinear dynamic monitoring is crucial for assessing l-tryptophan (l-Trp) dysregulation progression in tuberculous meningitis (TBM); yet remains challenging due to conventional sensing probes' inherent nondirectionality and poor interfacial response control. Herein, we present a programmable organic-ligand-based self-assembly strategy to construct superlattice nanoprobes with structurally responsive, tunable interfaces. Precisely regulating ligand assembly integrates atomically dispersed platinum layers with organic ligand bilayers, forming an interactive nanobiosensing interface for real-time, quantitative detection of nonlinear l-Trp dynamics. The nanoplatform shows threshold-dependent colorimetric response: subtle monochromatic brightness shifts below 0.2 μM, and distinct multiphase color transitions via chromatic superposition above it, enabling visual discrimination of pathological l-Trp fluctuations in TBM. Integrated with machine learning-enhanced pattern recognition, it achieves robust self-calibration and zero false positives in complex clinical matrices. Compared to conventional methods (acid-fast staining, culture), it reduces processing time by >50%, boosts accuracy by >13%, and cuts reagent costs by >74%, establishing an effective paradigm for neuroinflammatory biomarker monitoring and enabling precise TBM diagnosis.