A single microRNA miR-195 rescues the arrested B cell development induced by EBF1 deficiency.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 41649478.
- Also identified by DOI 10.7554/eLife.101510 and PMC identifier 12880805.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Accumulated studies have reported that hematopoietic differentiation was primarily regulated by transcription factors. Early B cell factor 1 (EBF1) is an essential transcription factor for B lymphopoiesis. Contrary to the canonical notion, we found that a single miRNA, miRNA-195 (<i>Mir195</i>) transduction let <i>Ebf1</i>-deficient hematopoietic progenitor cells (HPCs) express CD19, carry out V(D)J recombination and class switch recombination, which implied that B cell matured without EBF1. A part of the mechanism was caused by FOXO1 accumulation via inhibition of FOXO1 phosphorylation pathways in which targets of <i>Mir195</i> are enriched. These results suggested that some miRNA transductions could function as alternatives to transcription factors.
Medical subject headings
- MicroRNAs
- B-Lymphocytes
- Trans-Activators
- Cell Differentiation
- Lymphopoiesis