<i>map3k1</i> is required for spatial restriction of progenitor differentiation in planarians.

Abstract

Planarian regeneration and tissue turnover involve fate specification in pluripotent stem cells called neoblasts. Neoblasts select fates through the expression of fate-specific transcription factors, generating specialized neoblasts. Specialized neoblasts are spatially intermingled and can be dispersed broadly, frequently being present far from their target tissue. The post-mitotic progeny of neoblasts, serving as progenitors, can migrate and differentiate into mature cell types. Pattern formation is thus strongly influenced by the migratory assortment and differentiation of fate-specified progenitors in precise locations, which we refer to as progenitor targeting. This central step of pattern maintenance and formation, however, is poorly understood. Here, we describe a requirement for the conserved <i>map3k1</i> gene in targeting, restricting post-mitotic progenitor differentiation to precise locations. RNAi of <i>map3k1</i> causes ectopic differentiation of eye progenitors along their migratory path, resulting in dispersed, ectopic eye cells and eyes. Other neural tissues similarly display ectopic posterior differentiation, and ectopic pharynx cells emerge dispersed laterally and anteriorly in <i>map3k1</i> RNAi animals. Ectopic differentiated cells are also found within the incorrect organs after <i>map3k1</i> RNAi, and ultimately, teratomas form. These findings implicate <i>map3k1</i> signaling in controlling the positional regulation of progenitor behavior - restricting progenitor differentiation to targeted locations in response to external cues in the local tissue environment.