Alternative splicing-triggered mRNA decay informs splice-switching targets for neurodevelopmental disorders.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 41678398.
- Also identified by DOI 10.1172/JCI197271 and PMC identifier 13078869.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Alternative splicing-triggered nonsense-mediated mRNA decay (AS-NMD) critically regulates gene expression, but the extent to which neuronal genes are regulated by AS-NMD remains understudied. Here, we identified over 3,000 developmentally regulated AS-NMD exons in mouse and human brains and validated them in cultured neurons. AS-NMD suppresses synaptic genes during brain development and differentially regulates more than 200 causal genes for neurodevelopmental disorders (NDDs). We detected a poison exon in GRIA2 and identified splice-switching antisense oligonucleotides that suppressed GRIA2 NMD and increased its functional isoforms. In summary, this study uncovers genes repressed by AS-NMD in the brain and nominates amenable splice-switching targets for treating dominant NDDs such as autism spectrum disorders and developmental epileptic encephalopathy.
Medical subject headings
- Alternative Splicing
- Nonsense Mediated mRNA Decay
- Neurodevelopmental Disorders