Escaping bottlenecks: The demographic path to genetic recovery in koalas (<i>Phascolarctos cinereus</i>).

Abstract

Population bottlenecks can lead to evolutionary dead ends by eroding genetic diversity and intensifying inbreeding. Although theory predicts possible escape routes, direct observations of this process are rare. Using whole-genome data from 418 koalas, we found that populations with higher genetic diversity harbored the greatest mutational loads and had declining effective population sizes (<i>N</i>_e), whereas historically bottlenecked but recovering populations displayed reduced mutational load, exhibited increasing <i>N</i>_e, and regenerated new, rare variants. We concluded that this pattern was due to rapid demographic expansion, which reshuffled genetic variation through recombination and affected <i>N</i>_e more quickly than it did conventional diversity metrics. Our findings suggest that recovery of bottlenecked populations can occur through rapid demographic growth and that this can reestablish evolutionary potential in threatened populations.