PULPO: Pipeline of understanding large-scale patterns of oncogenomic signatures.

Portasany-Rodríguez, Marta; Soria-Alcaide, Gonzalo; Sánchez, Elena G; Ivanova, Mariya; Gómez, Ana; Giménez, Reyes; Lalchandani, Jaanam; García-Aguilera, Gonzalo et al. · Bioinformatics · 2026

basic_science · Level V

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Abstract

PULPO v1.0 is a novel, fully automated pipeline designed for the preprocess and extraction of mutational signatures from raw Optical Genome Mapping (OGM) data. Built using Snakemake and executed within an isolated, Conda-managed environment, PULPO transforms complex cytogenetic alterations, captured at ultra-high resolution, into Catalogue of somatic mutations in cancer mutational signatures (COSMIC). This innovative approach not only enables researchers to work directly from raw OGM inputs but also streamlines the traditionally complex process of signature extraction, making advanced oncogenomic analyses accessible to users with varying levels of bioinformatics expertise. By facilitating the integration of comprehensive structural variants (SVs) and copy number variants (CNVs) data with established signature catalogues, PULPO paves the way for improved diagnostic accuracy and personalized therapeutic strategies. The pipeline is open source and freely available under the MIT License at https://github.com/OncologyHNJ/PULPO-v.1.0 and DOI in Zenodo: https://zenodo.org/records/17749097. Supplementary data are available at Bioinformatics online.